Composite

Part:BBa_K2073010:Design

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


Crcb(flc) with IPTG promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 266
    Illegal AgeI site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the degradation of PFOA, fluoride would be released simultaneously. To prevent our E. coli get poisoned by excess fluoride, we transfected Crcb(flc) gene into it.
This biobrick can help our E. coli expulse fluoride so that them can survive and grow better.

Source

E.coli

References